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SMAP-29
General Information
DRACP ID DRACP00001
Peptide Name SMAP-29
Sequence RGLRRLGRKIAHGVKKYGPTVLRIIRIA
Sequence Length 28
UniProt ID Not available
PubChem CID 16130512
Origin Ovis aries
Type Native peptide
Classification
Active ACP Membrane-targeted
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50=30 µM | MTT assay | 2 days | 2 |
Hemolytic Activity Human erythrocytes (4% Hemolysis=4 µM; 19% Hemolysis=20 µM; 67% Hemolysis=80 µM; 50% Hemolysis=86 µM; 10% Hemolysis=6.0 µm) ; Sheep erythrocytes (3% Hemolysis=80 µM)
Normal (non-cancerous) Cytotoxicity NIH 3T3: IC50=16 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial; Antifungal
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C144H257N51O31
Absent amino acids CDEFMNQSW
Common amino acids R
Mass 368073
Pl 12.81
Basic residues 10
Acidic residues 0
Hydrophobic residues 11
Net charge 10
Boman Index -6364
Hydrophobicity -20.36
Aliphatic Index 125.36
Half Life
Mammalian: 1 hour
Yeast: 2 min
E.coli: 2 min
Extinction Coefficient cystines 1490
Absorbance 280nm 55.19
Polar residues 6
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 10601638
Title SMAP-29: a potent antibacterial and antifungal peptide from sheep leukocytes
Doi 10.1016/s0014-5793(99)01600-2
Year 1999
Literature 2
Pubmed ID 24221355
Title Bacterial killing mechanism of sheep myeloid antimicrobial peptide-18 (SMAP-18) and its Trp-substituted analog with improved cell selectivity and reduced mammalian cell toxicity
Year 2014
Literature 3
Pubmed ID 26795535
Title The stereochemical effect of SMAP-29 and SMAP-18 on bacterial selectivity, membrane interaction and anti-inflammatory activity
Year 2016
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DRAMP ID DRAMP02912
DBAASP ID 1485
This work is licensed under a Creative Commons Attribution 4.0 International License.