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CA-MA
General Information
DRACP ID DRACP00002
Peptide Name CA-MA
Sequence KWKLFKKIGIGKFLHSAKKF
Sequence Length 20
UniProt ID Not available
PubChem CID Not available
Origin Ceropin-Magainin hybrid peptides
Type Synthetic peptide
Classification
Active ACP Membrane-targeted
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | Leukemia | IC50=65 µM | MTT assay | 3 days | 1 |
| A549 | Lung adenocarcinoma | Carcinoma | IC50=60 µM | MTT assay | 3 days | 1 |
| Jurkat | Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia | Leukemia | IC50=30 µM | MTT assay | 3 days | 1 |
| MDA-MB-361 | Breast adenocarcinoma | Carcinoma | IC50=44 µM | MTT assay | 3 days | 1 |
| NCI-H128 | Lung small cell carcinoma; Small cell lung cancer | Carcinoma | IC50=16.1 µM | MTT assay | 72 h | 2 |
| NCI-H69 | Lung small cell carcinoma; Small cell lung cancer | Carcinoma | IC50=15.8 µM | MTT assay | 72 h | 2 |
| NCI-H146 | Lung small cell carcinoma; Small cell lung cancer | Carcinoma | IC50=15.8 µM | MTT assay | 72 h | 2 |
Hemolytic Activity Human erythrocytes (Hemolysis 0% =100 μM)
Normal (non-cancerous) Cytotoxicity NIH-3T3 fibroblast: IC50>100 μM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C120H190N30O22
Absent amino acids CDEMNPQRTVY
Common amino acids K
Mass 274410
Pl 11.58
Basic residues 8
Acidic residues 0
Hydrophobic residues 9
Net charge 8
Boman Index -1227
Hydrophobicity -31
Aliphatic Index 83
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 5500
Absorbance 280nm 289.47
Polar residues 3
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 10675500
Title Effects of the hinge region of cecropin A(1-8)-magainin 2(1-12), a synthetic antimicrobial peptide, on liposomes, bacterial and tumor cells
Doi 10.1016/s0005-2736(99)00210-2
Year 2000
Literature 2
Pubmed ID 9352466
Title Structure-antitumor and hemolytic activity relationships of synthetic peptides derived from cecropin A-magainin 2 and cecropin A-melittin hybrid peptides
Doi 10.1111/j.1399-3011.1997.tb01469.x
Year 1997
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.