HNP-3

General Information


DRACP ID  DRACP00024

Peptide Name   HNP-3

Sequence  DCYCRIPACIAGERRYGTCIYQGRLWAFCC

Sequence Length  30

UniProt ID  P59665  Q6EZE9 

PubChem CID  Not available

Origin  Human alpha-defensins

Type  Native peptide

Classification

  

Active ACP Membrane-targeted



Activity Information


Cell Line Disease Cancer Classified Activity Assay Testing Time Literature
RCCs (A-498, Caki-2, 786-0, 769-P, ACHN, LE 9211-RCC, TW33, N43) Renal Cell Carcinoma Carcinoma At higher concentrations than 25 μg/ml, HNPs-1, -2, and -3 exerted cytotoxic effects on all tested RCC lines in an in vitro serum-free culture system (at lower concentrations they stimulated cell grow WST-1 assay  40 h 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available

Nature  Anticancer



Structure Information


PDB ID  Not available

Predicted Structure  DRACP00024

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys2<--->Cys30; Cys4<--->Cys19; Cys9<--->Cys29

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C151H228N44O40S6

Absent amino acids  HKMNSV

Common amino acids  C

Mass  400958

Pl  8.11

Basic residues  4

Acidic residues  2

Hydrophobic residues  9

Net charge  2

Boman Index  -4282

Hydrophobicity  12.33

Aliphatic Index  62

Half Life 
  Mammalian: 1.2 hour
  Yeast: >20 hour
  E.coli: >10 hour

Extinction Coefficient cystines  10345

Absorbance 280nm  356.72

Polar residues  13

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 11943716

Title  Human alpha-defensins HNPs-1, -2, and -3 in renal cell carcinoma: influences on tumor cell proliferation

Doi 10.1016/s0002-9440(10)62558-8

Year  2002

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




CancerPPD ID  4432

DRACP is developed by Dr.Zheng's team.