N-1

General Information


DRACP ID  DRACP00029

Peptide Name   N-1

Sequence  WKLFKKIPKFLHLAKKF

Sequence Length  17

UniProt ID  Not available

PubChem CID  Not available

Origin  Ceropin A

Type  Synthetic peptide

Classification

  

Active ACP Membrane-targeted



Activity Information


Cell Line Disease Cancer Classified Activity Assay Testing Time Literature
MDA-MB-361 Breast adenocarcinoma Carcinoma IC50=9.5 µM MTT assay 3 days 1
Jurkat Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia Leukemia IC50=12 µM MTT assay 3 days 1
K562 Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia Leukemia IC50=6 µM MTT assay 3 days 1

Hemolytic Activity  Human erythrocytes: hemolysis: 10.1%=100 µM; hemolysis: 3%=50 µM; hemolysis: 0.7%=25.0 µM; hemolysis: 0%=12.5 µM; hemolysis: 0%=6.25 µM; hemolysis: 0%=3.125 µM; hemolysis: 0%=1.56 µM; hemolysis: 0%=0.78 µM

Normal (non-cancerous) Cytotoxicity  NIH 3T3: IC50=75 µM

Target  Not available

Affinity  Not available

Mechanism  Not available

Nature  Anticancer; Antibacterial



Structure Information


PDB ID  Not available

Predicted Structure  DRACP00029

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C112H174N26O18

Absent amino acids  CDEGMNQRSTVY

Common amino acids  K

Mass  245835

Pl  11.5

Basic residues  7

Acidic residues  0

Hydrophobic residues  9

Net charge  7

Boman Index  -520

Hydrophobicity  -17.65

Aliphatic Index  97.65

Half Life 
  Mammalian: 1.3 hour
  Yeast: 3 min
  E.coli: 2 min

Extinction Coefficient cystines  5500

Absorbance 280nm  343.75

Polar residues  0

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 12005420

Title  Antibacterial, antitumor and hemolytic activities of alpha-helical antibiotic peptide, P18 and its analogs

Doi 10.1034/j.1399-3011.2001.00934.x

Year  2001

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DRACP is developed by Dr.Zheng's team.