This work is licensed under a Creative Commons Attribution 4.0 International License.
Temporin-1Tl
General Information
DRACP ID DRACP00048
Peptide Name Temporin-1Tl
Sequence FVQWFSKFLGRIL
Sequence Length 13
UniProt ID Not available
PubChem CID Not available
Origin Rana temporaria
Type Native peptide
Classification
Active ACP Membrane-targeted
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| Hut-78 | Sezary syndrome | Lymphoma | 100% Killing=10 µM | Trypan blue assay | Not available | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL9 positive; Chronic myeloid leukemia | Leukemia | 60% Killing=10 µM | Trypan blue assay | Not available | 1 |
| U-937 | Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia | Leukemia | 20% Killing=10 µM | Trypan blue assay | Not available | 1 |
Hemolytic Activity Human erythrocytes: 94% Hemolysis=24 µM; 92% Hemolysis=12 µM; 48% Hemolysis=6 µM; 13% Hemolysis=3 µM; 3% Hemolysis=1.5 µM; 100% Hemolysis=40 µM
Normal (non-cancerous) Cytotoxicity NIH 3T3: 100% Killing=40 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial; Antifungal
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C83H121N19O16
Absent amino acids ACDEHMNPTY
Common amino acids F
Mass 185521
Pl 11.65
Basic residues 2
Acidic residues 0
Hydrophobic residues 8
Net charge 2
Boman Index 160
Hydrophobicity 82.31
Aliphatic Index 112.31
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 5500
Absorbance 280nm 458.33
Polar residues 2
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 12133008
Title Temporin L: antimicrobial, haemolytic and cytotoxic activities, and effects on membrane permeabilization in lipid vesicles
Year 2002
Literature 2
Pubmed ID 21319749
Title Structure-activity relationship, conformational and biological studies of temporin L analogues
Year 2011
Literature 3
Pubmed ID 32786286
Title A Noncytotoxic Temporin L Analogue with In Vivo Antibacterial and Antiendotoxin Activities and a Nonmembrane-Lytic Mode of Action
Doi 10.1021/acsinfecdis.0c00022
Year 2020
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.