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Varv H
General Information
DRACP ID DRACP00074
Peptide Name Varv H
Sequence GLPVCGETCFGGTCNTPGCSCETWPVCSRN
Sequence Length 30
UniProt ID P58453
PubChem CID Not available
Origin Viola arvensis (European field pansy) (Field violet)
Type Native peptide
Classification
Active ACP Membrane-targeted
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| U251 | Astrocytoma | Carcinoma | IC50=44.70 µg/ml | SRB assay | 48h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | Carcinoma | IC50>10 µg/ml | SRB assay | 48h | 1 |
| A549 | Lung adenocarcinoma | Carcinoma | IC50>10 µg/ml | SRB assay | 48h | 1 |
| DU145 | Prostate carcinoma | Carcinoma | IC50>10 µg/ml | SRB assay | 48h | 1 |
| BEL-7402 | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50>10 µg/ml | SRB assay | 48h | 1 |
Hemolytic Activity HRBCs: HD50=7.52 µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys27; NCB: Gly1<--->Asn30
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C125H192N36O43S6
Absent amino acids ADHIKMQY
Common amino acids C
Mass 359692
Pl 4.26
Basic residues 1
Acidic residues 2
Hydrophobic residues 5
Net charge -1
Boman Index -2821
Hydrophobicity -2
Aliphatic Index 32.33
Half Life
Mammalian: 30 hour
Yeast: >20 hour
E.coli: >10 hour
Extinction Coefficient cystines 5875
Absorbance 280nm 202.59
Polar residues 19
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 20580652
Title Isolation and characterization of cytotoxic cyclotides from Viola tricolor
Doi 10.1016/j.peptides.2010.05.004
Year 2010
Literature 2
Pubmed ID 10600388
Title Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif
Year 1999
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.