Varv D

General Information


DRACP ID  DRACP00075

Peptide Name   Varv D

Sequence  GLPICGETCVGGSCNTPGCSCSWPVCTRN

Sequence Length  29

UniProt ID  P58449 

PubChem CID  Not available

Origin  Viola arvensis (European field pansy) (Field violet)

Type  Native peptide

Classification

  

Active ACP Membrane-targeted



Activity Information


Cell Line Disease Cancer Classified Activity Assay Testing Time Literature
U251 Astrocytoma Carcinoma IC50=46.62 µg/ml SRB assay 48h 1
MDA-MB-231 Breast adenocarcinoma Carcinoma IC50>10 µg/ml SRB assay 48h 1
A549 Lung adenocarcinoma Carcinoma IC50>10 µg/ml SRB assay 48h 1
DU145 Prostate carcinoma Carcinoma IC50>10 µg/ml SRB assay 48h 1
BEL-7402 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50>10 µg/ml SRB assay 48h 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available

Nature  Anticancer



Structure Information


PDB ID  Not available

Predicted Structure  DRACP00075

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asn29

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C116H185N35O40S6

Absent amino acids  ADFHKMQY

Common amino acids  C

Mass  340193

Pl  5.94

Basic residues  1

Acidic residues  1

Hydrophobic residues  5

Net charge  0

Boman Index  -2029

Hydrophobicity  15.52

Aliphatic Index  46.9

Half Life 
  Mammalian: 30 hour
  Yeast: >20 hour
  E.coli: >10 hour

Extinction Coefficient cystines  5875

Absorbance 280nm  209.82

Polar residues  19

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 20580652

Title  Isolation and characterization of cytotoxic cyclotides from Viola tricolor

Doi 10.1016/j.peptides.2010.05.004

Year  2010

Literature 2

Pubmed ID 10600388

Title  Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif

Doi 10.1006/jmbi.1999.3383

Year  1999

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DRACP is developed by Dr.Zheng's team.