This work is licensed under a Creative Commons Attribution 4.0 International License.
NK-2
General Information
DRACP ID DRACP00147
Peptide Name NK-2
Sequence KILRGVCKKIMRTFLRRISKDILTGKK
Sequence Length 27
UniProt ID Q29075
PubChem CID Not available
Origin Sus scrofa (Pig)
Type Native peptide
Classification
Active ACP Membrane lysis
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| LA-N-1 | Neuroblastoma | Blastoma | LD50=1-4 µM | PI-uptake assay | Not available | 1 |
| SH-SY5Y | Neuroblastoma | Blastoma | LD50=1-4 µM | PI-uptake assay | Not available | 1 |
| U-937 | Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia | Leukemia | LD50=30 µM | PI-uptake assay | Not available | 1 |
| SW480 | Colon adenocarcinoma | Carcinoma | LD50=1-4 µM | PI-uptake assay | Not available | 1 |
| E42/02 | Horse sarcoid | Sarcoma | IC50=~70 µM | MTT assay | 30 min | 2 |
| E42/02 | Horse sarcoid | Sarcoma | IC50=~50 µM | MTT assay | 2 h | 2 |
| E42/02 | Horse sarcoid | Sarcoma | IC50=~50 µM | MTT assay | 4 h | 2 |
| E42/02 | Horse sarcoid | Sarcoma | IC50=~40 µM | MTT assay | 24 h | 2 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | 28.7 ± 9.3% Cytotoxicity=100 μg/ml | MTT assay | 24 h | 3 |
Hemolytic Activity Human erythrocytes: 45% Hemolysis=100 µg/ml
Normal (non-cancerous) Cytotoxicity Non-toxic to a human keratinocyte cell line
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C143H261N45O33S2
Absent amino acids AEHNPQWY
Common amino acids K
Mass 366699
Pl 12.16
Basic residues 10
Acidic residues 1
Hydrophobic residues 9
Net charge 9
Boman Index -6327
Hydrophobicity -26.3
Aliphatic Index 111.85
Half Life
Mammalian: 20 hour
Yeast: 30 min
E.coli: >10 hour
Extinction Coefficient cystines 0
Absorbance 280nm 0
Polar residues 6
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 16269280
Title The NK-lysin derived peptide NK-2 preferentially kills cancer cells with increased surface levels of negatively charged phosphatidylserine
Doi 10.1016/j.febslet.2005.09.084
Year 2005
Literature 2
Pubmed ID 23893605
Title Design of NK-2-derived peptides with improved activity against equine sarcoid cells
Doi 10.1002/psc.2540
Year 2013
Literature 3
Pubmed ID 17389605
Title Rationale for the design of shortened derivatives of the NK-lysin-derived antimicrobial peptide NK-2 with improved activity against Gram-negative pathogens
Year 2007
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.