This work is licensed under a Creative Commons Attribution 4.0 International License.
Brevinin-1DYb
General Information
DRACP ID DRACP00149
Peptide Name Brevinin-1DYb
Sequence FLSLALAALPKLFCLIFKKC
Sequence Length 20
UniProt ID Not available
PubChem CID Not available
Origin Rana dybowskii; Rana pirica; Rana amurensis
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| RT-4(G2) | Prostate tumor | Carcinoma | IC50=32.3 µM | LDH leakage assay | 48 h | 1 |
| 647V | Bladder carcinoma | Carcinoma | IC50=54.1 µM | LDH leakage assay | 48 h | 1 |
| 486P | Bladder carcinoma | Carcinoma | IC50=28.6 µM | LDH leakage assay | 48 h | 1 |
| RT-4(G2) | Prostate tumor | Carcinoma | IC50=484 µM | WST-1 assay | 48 h | 1 |
| 647V | Bladder carcinoma | Carcinoma | IC50=52.4 µM | WST-1 assay | 48 h | 1 |
| 486P | Bladder carcinoma | Carcinoma | IC50=57.8 µM | WST-1 assay | 48 h | 1 |
| RT-4(G2) | Prostate tumor | Carcinoma | IC50=135.3 µM | Cell Viability assay | 48 h | 1 |
| 647V | Bladder carcinoma | Carcinoma | IC50=31 µM | Cell Viability assay | 48 h | 1 |
| 486P | Bladder carcinoma | Carcinoma | IC50=59.4 µM | Cell Viability assay | 48 h | 1 |
| NCI-H157 | Lung squamous cell carcinoma | Carcinoma | IC50=4.5 µM | MTT assay | Not available | 1 |
| PC-3 | Prostate carcinoma | Carcinoma | IC50=6.4 µM | MTT assay | Not available | 1 |
| U-251MG | Astrocytoma | Carcinoma | IC50=4.6 µM | MTT assay | Not available | 1 |
| MDA-MB-435S | Amelanotic melanoma | Carcinoma | IC50=9.5 µM | MTT assay | Not available | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | IC50=9.1 µM | MTT assay | Not available | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis<5 µM; 50% Hemolysis=125 µM; 50% Hemolysis=7 µM; Horse erythrocytes: 50% Hemolysis=4.7 µM
Normal (non-cancerous) Cytotoxicity HMEC-1: IC50=25.2 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial; Antifungal
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys14<--->Cys20
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C110H179N23O22S2
Absent amino acids DEGHMNQRTVWY
Common amino acids L
Mass 257900
Pl 9.67
Basic residues 3
Acidic residues 0
Hydrophobic residues 13
Net charge 3
Boman Index 3132
Hydrophobicity 160
Aliphatic Index 151.5
Half Life
Mammalian: 5.5 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 125
Absorbance 280nm 6.58
Polar residues 3
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 29366784
Title Identification of novel Amurin-2 variants from the skin secretion of Rana amurensis, and the design of cationicity-enhanced analogues
Doi 10.1016/j.bbrc.2018.01.124
Year 2018
Literature 2
Pubmed ID 17688900
Title Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii
Doi 10.1016/j.toxicon.2007.06.023
Year 2007
Literature 3
Pubmed ID 19539775
Title Characterization of antimicrobial peptides isolated from the skin of the Chinese frog, Rana dybowskii
Doi 10.1016/j.cbpb.2009.05.015
Year 2009
Literature 4
Pubmed ID 15003829
Title A family of brevinin-2 peptides with potent activity against Pseudomonas aeruginosa from the skin of the Hokkaido frog, Rana pirica
Doi 10.1016/j.regpep.2003.12.003
Year 2004
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.