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Lactoferricin B
General Information
DRACP ID DRACP00153
Peptide Name Lactoferricin B
Sequence FKCRRWQWRMKKLGAPSITCVRRAF
Sequence Length 25
UniProt ID P24627
PubChem CID Not available
Origin The big centipede Scolopendra subspinipes mutilans
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| Kelly | Neuroblastoma | Blastoma | IC50=15.5 µM | MTT assay | 48 h | 1 |
| IMR-32 | Neuroblastoma | Blastoma | IC50=29 µM | MTT assay | 48 h | 1 |
| SK-N-DZ | Neuroblastoma | Blastoma | IC50=37 µM | MTT assay | 48 h | 1 |
| SHEP-12 | Neuroblastoma | Blastoma | IC50=45 µM | MTT assay | 48 h | 1 |
| SH-SY5Y | Neuroblastoma | Blastoma | IC50=60 µM | MTT assay | 48 h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism LfcinB possesses a cytotoxic effect on MYCN-amplified NB cells by directly targeting and destabilizing the cytoplasmic membrane and the mitochondria.
Nature Anticancer; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys3 <--> Cys20
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C141H226N46O29S3
Absent amino acids DEHNY
Common amino acids R
Mass 355407
Pl 12.35
Basic residues 8
Acidic residues 0
Hydrophobic residues 9
Net charge 8
Boman Index -6879
Hydrophobicity -57.6
Aliphatic Index 50.8
Half Life
Mammalian:
Yeast:
E.coli:
Extinction Coefficient cystines 11125
Absorbance 280nm 463.54
Polar residues 5
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 16572423
Title The antimicrobial peptide, lactoferricin B, is cytotoxic to neuroblastoma cells in vitro and inhibits xenograft growth in vivo
Year 2006
Literature 2
Pubmed ID 11908643
Title Towards a structure-function analysis of bovine lactoferricin and related tryptophan- and arginine-containing peptides
Doi 10.1139/o01-213
Year 2002
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.