This work is licensed under a Creative Commons Attribution 4.0 International License.
Brevinin-2R
General Information
DRACP ID DRACP00196
Peptide Name Brevinin-2R
Sequence KLKNFAIGVAQSLLNKASCKLSGQC
Sequence Length 25
UniProt ID P85095
PubChem CID Not available
Origin Pelophylax ridibundus (Marsh frog) (Rana ridibunda)
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | LD50=10-15μg/ml | MTT assay | 4h | 1 |
| Jurkat | Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia | Leukemia | LD50=20-25μg/ml | MTT assay | 4h | 1 |
| BJAB | Burkitt lymphoma | Lymphoma | LD50=30-40μg/ml | MTT assay | 4h | 1 |
Hemolytic Activity Sheep erythrocytes(2.5% hemolytic activity at 200 μg/ml)
Normal (non-cancerous) Cytotoxicity L929: LD50=20-25μg/ml
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticance; Antimicrobial; Antibacterial
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Not available
C-terminal Modification Not available
Other Modification None
Chiral L
Physicochemical Information
Formula C114H197N33O33S2
Absent amino acids DEHMPRTWY
Common amino acids KL
Mass 305043
Pl 10.35
Basic residues 4
Acidic residues 0
Hydrophobic residues 10
Net charge 4
Boman Index -1527
Hydrophobicity 17.2
Aliphatic Index 101.6
Half Life
Mammalian: 1 hour
Yeast: 2 min
E.coli: 2 min
Extinction Coefficient cystines 125
Absorbance 280nm 5.21
Polar residues 9
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 18494941
Title Brevinin-2R(1) semi-selectively kills cancer cells by a distinct mechanism, which involves the lysosomal-mitochondrial death pathway
Doi 10.1111/j.1582-4934.2008.00129.x
Year 2008
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.