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Temporin-CEa

General Information

DRACP ID  DRACP00212

Peptide Name   Temporin-CEa

Sequence  FVDLKKIANIINSIF

Sequence Length  15

UniProt ID  Not available

PubChem CID  Not available

Origin  skin of the Chinese brown frog, Rana chensinensis

Type  Native peptide

Classification

  

Active ACP



Activity Information

Cell Line Disease Cancer Classified Activity Assay Testing Time Literature
MCF-7 Invasive breast carcinoma of no special type Carcinoma IC50=12 µM MTT assay 24 h 1
SMMC-7721 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=44.92 μM MTT assay 1 h 2
BEL-7402 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=36.9 μM MTT assay 1 h 2
BCaP-37 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=37.33 μM MTT assay 1 h 2
MDA-MB-231 Breast adenocarcinoma Carcinoma IC50=63.26 μM MTT assay 1 h 2
MCF-7 Invasive breast carcinoma of no special type Carcinoma IC50=31.78 μM MTT assay 1 h 2
LK-2 Lung squamous cell carcinoma Carcinoma IC50=58.32 μM MTT assay 1 h 2
A549 Lung adenocarcinoma Carcinoma IC50=52.82 μM MTT assay 1 h 2
NCI-H446 Lung small cell carcinoma; Small cell lung cancer Carcinoma IC50=59.45 μM MTT assay 1 h 2
BGC-823 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=56.47 μM MTT assay 1 h 2
HeLa Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=43.87 μM MTT assay 1 h 2
HO-8910 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=62.84 μM MTT assay 1 h 2
HT-29 Colon adenocarcinoma Carcinoma IC50=88.91 μM MTT assay 1 h 2
SMMC-7721 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=46.73 μM MTT assay 6 h 2
BEL-7402 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=37.1 μM MTT assay 6 h 2
BCaP-37 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=38.5 μM MTT assay 6 h 2
MDA-MB-231 Breast adenocarcinoma Carcinoma IC50=60.38 μM MTT assay 6 h 2
MCF-7 Invasive breast carcinoma of no special type Carcinoma IC50=27.84 μM MTT assay 6 h 2
LK-2 Lung squamous cell carcinoma Carcinoma IC50=61.73 μM MTT assay 6 h 2
A549 Lung adenocarcinoma Carcinoma IC50=53.12 μM MTT assay 6 h 2
NCI-H446 Lung small cell carcinoma; Small cell lung cancer Carcinoma IC50=64.81 μM MTT assay 6 h 2
BGC-823 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=55.16 μM MTT assay 6 h 2
HeLa Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=40.6 μM MTT assay 6 h 2
HO-8910 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=66.68 μM MTT assay 6 h 2
HT-29 Colon adenocarcinoma Carcinoma IC50>100 μM MTT assay 6 h 2
SMMC-7721 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=50.32 μM MTT assay 24 h 2
BEL-7402 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=38.98 μM MTT assay 24 h 2
BCaP-37 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=39.42 μM MTT assay 24 h 2
MDA-MB-231 Breast adenocarcinoma Carcinoma IC50=57.94 μM MTT assay 24 h 2
MCF-7 Invasive breast carcinoma of no special type Carcinoma IC50=31.91 μM MTT assay 24 h 2
LK-2 Lung squamous cell carcinoma Carcinoma IC50=63.4 μM MTT assay 24 h 2
A549 Lung adenocarcinoma Carcinoma IC50=53.31 μM MTT assay 24 h 2
NCI-H446 Lung small cell carcinoma; Small cell lung cancer Carcinoma IC50=67.66 μM MTT assay 24 h 2
BGC-823 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=61.63 μM MTT assay 24 h 2
HeLa Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=36.31 μM MTT assay 24 h 2
HO-8910 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=64.75 μM MTT assay 24 h 2
HT-29 Colon adenocarcinoma Carcinoma IC50>100 μM MTT assay 24 h 2
SMMC-7721 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=47.73 μM MTT assay 48 h 2
BEL-7402 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=40.16 μM MTT assay 48 h 2
BCaP-37 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=38.39 μM MTT assay 48 h 2
MDA-MB-231 Breast adenocarcinoma Carcinoma IC50=54.95 μM MTT assay 48 h 2
MCF-7 Invasive breast carcinoma of no special type Carcinoma IC50=34.5 μM MTT assay 48 h 2
LK-2 Lung squamous cell carcinoma Carcinoma IC50=61.57 μM MTT assay 48 h 2
A549 Lung adenocarcinoma Carcinoma IC50=52.09 μM MTT assay 48 h 2
NCI-H446 Lung small cell carcinoma; Small cell lung cancer Carcinoma IC50=65.29 μM MTT assay 48 h 2
BGC-823 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=63.2 μM MTT assay 48 h 2
HeLa Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=38.96 μM MTT assay 48 h 2
HO-8910 Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=66.55 μM MTT assay 48 h 2
HT-29 Colon adenocarcinoma Carcinoma IC50>100 μM MTT assay 48 h 2

Hemolytic Activity  Human erythrocytes: LD50=230 μM

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available

Nature  Anticancer



Structure Information

PDB ID  Not available

Predicted Structure  DRACP00212

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information

Formula  C83H135N19O21

Absent amino acids  CEGHMPQRTWY

Common amino acids  I

Mass  198501

Pl  9.54

Basic residues  2

Acidic residues  1

Hydrophobic residues  9

Net charge  1

Boman Index  -9

Hydrophobicity  95.33

Aliphatic Index  156

Half Life 
  Mammalian: 1.1 hour
  Yeast: 3 min
  E.coli: 2 min

Extinction Coefficient cystines  0

Absorbance 280nm  0

Polar residues  3

Amino acid distribution



Literature Information

Literature 1

Pubmed ID 19341344

Title  Molecular cloning of cDNAs encoding antimicrobial peptide precursors from the skin of the Chinese brown frog, Rana chensinensis

Doi 10.2108/zsj.26.220

Year  2009

Literature 2

Pubmed ID 21871946

Title  Antitumor effects and cell selectivity of temporin-1CEa, an antimicrobial peptide from the skin secretions of the Chinese brown frog (Rana chensinensis)

Doi 10.1016/j.biochi.2011.08.011

Year  2012

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License.

DRACP is developed by Dr.Zheng's team.