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Camel
General Information
DRACP ID DRACP00231
Peptide Name Camel
Sequence KWKLFKKIGAVLKVL
Sequence Length 15
UniProt ID Not available
PubChem CID Not available
Origin Ceropin-Melittin hybrid
Type Synthetic peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| B16-F10 | Mouse melanoma | Carcinoma | LC50=1.75 µM | MTT assay | 24 h | 1 |
| RenCa | Mouse kidney carcinoma | Carcinoma | LC50=1.3 µM | MTT assay | 24 h | 1 |
| 4T1 | Malignant neoplasms of the mouse mammary gland | Carcinoma | LC50=6.2 µM | MTT assay | 24 h | 1 |
| WM1158 | Melanoma | Carcinoma | LC50=4.1 µM | MTT assay | 24 h | 1 |
Hemolytic Activity At CAMEL concentrations toxic for B16-F10 cells the peptide did not cause erythrocyte hemolysis.
Normal (non-cancerous) Cytotoxicity Increasing the amount of CAMEL (1–20 μM) proved toxic to all tested cell lines The LC50 values were from 2.95 to 5.2 μM for normal cells (HECa10, HEC PP, NIH3T3, Wi38)
Target Not available
Affinity Not available
Mechanism specific combinations of necrosis-inducing peptide (CAMEL) and IL-12-carrying plasmid DNA can inhibit tumor growth as well as prevent tumor relapse
Nature Anticancer; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C89H151N21O16
Absent amino acids CDEHMNPQRSTY
Common amino acids K
Mass 202122
Pl 11.4
Basic residues 5
Acidic residues 0
Hydrophobic residues 9
Net charge 5
Boman Index 807
Hydrophobicity 54
Aliphatic Index 149.33
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 5500
Absorbance 280nm 392.86
Polar residues 1
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 20212453
Title Anticancer effects of CAMEL peptide
Year 2010
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.