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A12L
General Information
DRACP ID DRACP00253
Peptide Name A12L
Sequence KWKSFLKTFKSLKKTVLHTALKAISS
Sequence Length 26
UniProt ID Not available
PubChem CID Not available
Origin PeptideV13K
Type Synthetic peptide
Classification
Active ACP Membrane-targeted
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| A549 | Lung adenocarcinoma | Carcinoma | IC50=11.1 μmol/L | MTT assay | 36 h | 1 |
| A-375 | Amelanotic melanoma | Carcinoma | IC50=4.3 μmol/L | MTT assay | 36 h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | IC50=5 μmol/L | MTT assay | 36 h | 1 |
| SW1116 | Colon adenocarcinoma | Carcinoma | IC50=6.4 μmol/L | MTT assay | 36 h | 1 |
| RD | Embryonal rhabdomyosarcoma | Sarcoma | IC50=7.5 μmol/L | MTT assay | 36 h | 1 |
| NCI-H1299 | Lung large cell carcinoma | Carcinoma | IC50=4.4 μmol/L | MTT assay | 36 h | 1 |
| B16 | Mouse melanoma | Carcinoma | IC50=5.2 μmol/L | MTT assay | 36 h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50=3.8 μmol/L | MTT assay | 36 h | 1 |
Hemolytic Activity Human red blood cells: MHC=41.8 ± 0.30 μmol/L
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Peptides killed cancer cells with a fast necrotic mechanism causing cell membrane lysis
Nature Anticancer
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C142H236N36O34
Absent amino acids CDEGMNPQRY
Common amino acids K
Mass 343775
Pl 11.58
Basic residues 8
Acidic residues 0
Hydrophobic residues 11
Net charge 8
Boman Index -2427
Hydrophobicity -13.85
Aliphatic Index 93.85
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 5500
Absorbance 280nm 220
Polar residues 7
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 21252288
Title Studies on mechanism of action of anticancer peptides by modulation of hydrophobicity within a defined structural framework
Doi 10.1158/1535-7163.MCT-10-0811
Year 2011
Literature 2
Pubmed ID 15677462
Title Rational design of alpha-helical antimicrobial peptides with enhanced activities and specificity/therapeutic index
Year 2005
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.