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A12L/A20L/A23L
General Information
DRACP ID DRACP00256
Peptide Name A12L/A20L/A23L
Sequence KWKSFLKTFKSLKKTVLHTLLKLISS
Sequence Length 26
UniProt ID Not available
PubChem CID Not available
Origin PeptideV13K
Type Synthetic peptide
Classification
Active ACP Membrane-targeted
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| A549 | Lung adenocarcinoma | Carcinoma | IC50=9.2 μmol/L | MTT assay | 36 h | 1 |
| A-375 | Amelanotic melanoma | Carcinoma | IC50=7.2 μmol/L | MTT assay | 36 h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | IC50=5.7 μmol/L | MTT assay | 36 h | 1 |
| SW1116 | Colon adenocarcinoma | Carcinoma | IC50=3.5 μmol/L | MTT assay | 36 h | 1 |
| RD | Embryonal rhabdomyosarcoma | Sarcoma | IC50=5.5 μmol/L | MTT assay | 36 h | 1 |
| NCI-H1299 | Lung large cell carcinoma | Carcinoma | IC50=2.7 μmol/L | MTT assay | 36 h | 1 |
| B16 | Mouse melanoma | Carcinoma | IC50=4.6 μmol/L | MTT assay | 36 h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50=2.2 μmol/L | MTT assay | 36 h | 1 |
Hemolytic Activity Human red blood cells: MHC=2.6 ± 0.01 μmol/L
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Peptides killed cancer cells with a fast necrotic mechanism causing cell membrane lysis
Nature Anticancer
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C148H248N36O34
Absent amino acids ACDEGMNPQRY
Common amino acids K
Mass 352191
Pl 11.58
Basic residues 8
Acidic residues 0
Hydrophobic residues 11
Net charge 8
Boman Index -1805
Hydrophobicity 1.54
Aliphatic Index 116.15
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 5500
Absorbance 280nm 220
Polar residues 7
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 21252288
Title Studies on mechanism of action of anticancer peptides by modulation of hydrophobicity within a defined structural framework
Doi 10.1158/1535-7163.MCT-10-0811
Year 2011
Literature 2
Pubmed ID 15677462
Title Rational design of alpha-helical antimicrobial peptides with enhanced activities and specificity/therapeutic index
Year 2005
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.