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NRC-07
General Information
DRACP ID DRACP00323
Peptide Name NRC-07
Sequence RWGKWFKKATHVGKHVGKAALTAYL
Sequence Length 25
UniProt ID Not available
PubChem CID Not available
Origin Pleurocidin-family
Type Native peptide
Classification
Active ACP Cancer targeted peptides
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | Leukemia | 50% Cell death=16 µg/ml | MTT assay | 2h | 1 |
Hemolytic Activity 25 μM NRC-07: HMECs Cytotoxicity=20%, Fibroblasts Cytotoxicity=1%, HUVECs Cytotoxicity=17%; 50 μM NRC-07: HMECs Cytotoxicity=47%, Fibroblasts Cytotoxicity=0%, HUVECs Cytotoxicity=17%
Normal (non-cancerous) Cytotoxicity Erythrocytes(25 μM NRC-07): Hemolysis=2%; Erythrocytes(50 μM NRC-07): Hemolysis=3%;
Target Cell surface molecules
Affinity Not available
Mechanism NRC-03- and NRC-07-mediated cell death is initiated by peptide binding to negatively-charged molecules
Nature Anticancer; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Biotinylated
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C136H209N39O29
Absent amino acids CDEIMNPQS
Common amino acids K
Mass 328268
Pl 11.39
Basic residues 8
Acidic residues 0
Hydrophobic residues 11
Net charge 8
Boman Index -2165
Hydrophobicity -40.4
Aliphatic Index 70.4
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 12490
Absorbance 280nm 520.42
Polar residues 6
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 22023734
Title Pleurocidin-family cationic antimicrobial peptides are cytolytic for breast carcinoma cells and prevent growth of tumor xenografts
Doi 10.1186/bcr3043
Year 2011
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.