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RGD-La
General Information
DRACP ID DRACP00406
Peptide Name RGD-La
Sequence RGDLLRHVVKILEKYL
Sequence Length 16
UniProt ID Not available
PubChem CID Not available
Origin Arg-Gly-Asp(RGD) tripeptide ana temporins
Type Synthetic peptide
Classification
Active ACP Membrane-targeted
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| SW1116 | Colon adenocarcinoma | Carcinoma | 72.69 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| SMMC-7721 | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | 70.39 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | 73.09 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| Hep-G2 | Hepatoblastoma; Hepatoblastoma | Blastoma | 60.22 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| BGC-823 | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | 62.49 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| A549 | Lung adenocarcinoma | Carcinoma | 47.26Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| HL-7702(L-02) | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | 36.25 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| MEC | Cholangiocarcinoma; Cholangiocarcinoma | Carcinoma | 19.76 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
Hemolytic Activity Showed no hemolytic activity against rabbit erythrocytes and human erythrocytes at 250 µg/ml
Normal (non-cancerous) Cytotoxicity Showed little effects on the proliferation of the 293T human embryonic kidney cell line, MECs, and human lymphomonocytes at concentrations of less than 50 µg/ml
Target Not available
Affinity Not available
Mechanism A membrane-disturbing action seems to be the major mechanism for cell death
Nature Anticancer
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C90H154N26O22
Absent amino acids ACFMNPQSTW
Common amino acids L
Mass 222012
Pl 10.24
Basic residues 5
Acidic residues 2
Hydrophobic residues 7
Net charge 3
Boman Index -2765
Hydrophobicity -3.75
Aliphatic Index 158.13
Half Life
Mammalian: 5.5 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 1490
Absorbance 280nm 99.33
Polar residues 2
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 22641352
Title Designed synthetic analogs of the α-helical peptide temporin-La with improved antitumor efficacies via charge modification and incorporation of the integrin αvβ3 homing domain
Doi 10.1002/psc.2420
Year 2012
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.