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RGD-Las
General Information
DRACP ID DRACP00407
Peptide Name RGD-Las
Sequence RGDLLRHVVKILSKYL
Sequence Length 16
UniProt ID Not available
PubChem CID Not available
Origin Arg-Gly-Asp(RGD) tripeptide ana temporins
Type Synthetic peptide
Classification
Active ACP Membrane-targeted
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| SW1116 | Colon adenocarcinoma | Carcinoma | 81.465 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| SMMC-7721 | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | 86.175 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | 78.045 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| Hep-G2 | Hepatoblastoma; Hepatoblastoma | Blastoma | 73.07 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| BGC-823 | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | 73.705 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| A549 | Lung adenocarcinoma | Carcinoma | 52.50 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| HL-7702(L-02) | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | 38.13 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
| MEC | Cholangiocarcinoma; Cholangiocarcinoma | Carcinoma | 29.33 Inhibition ratio at 50 µg/ml | MTT assay | 24 h | 1 |
Hemolytic Activity Showed no hemolytic activity against rabbit erythrocytes and human erythrocytes at 250 µg/ml
Normal (non-cancerous) Cytotoxicity Showed little effects on the proliferation of the 293T human embryonic kidney cell line, MECs, and human lymphomonocytes at concentrations of less than 50 µg/ml
Target Not available
Affinity Not available
Mechanism A membrane-disturbing action seems to be the major mechanism for cell death
Nature Anticancer
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C88H152N26O21
Absent amino acids ACEFMNPQTW
Common amino acids L
Mass 217808
Pl 10.89
Basic residues 5
Acidic residues 1
Hydrophobic residues 7
Net charge 4
Boman Index -2424
Hydrophobicity 13.13
Aliphatic Index 158.13
Half Life
Mammalian: 1 hour
Yeast: 2 min
E.coli: 2 min
Extinction Coefficient cystines 1490
Absorbance 280nm 99.33
Polar residues 3
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 22641352
Title Designed synthetic analogs of the α-helical peptide temporin-La with improved antitumor efficacies via charge modification and incorporation of the integrin αvβ3 homing domain
Doi 10.1002/psc.2420
Year 2012
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.