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Melittin
General Information
DRACP ID DRACP00468
Peptide Name Melittin
Sequence GIGAVLKVLTTGLPALISWIKRKRQQ
Sequence Length 26
UniProt ID P68407 P01501 Q8LW54
PubChem CID Not available
Origin Apis mellifera (Honeybee)
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| E42/02 | Horse sarcoid | Sarcoma | IC50=3.2 ± 0.1 µM | MTT assay | 30 min | 1 |
| E42/02 | Horse sarcoid | Sarcoma | IC50=2.1 ± 0.1 µM | MTT assay | 2 h | 1 |
| E42/02 | Horse sarcoid | Sarcoma | IC50=1.8 ± 0.1 µM | MTT assay | 4 h | 1 |
| E42/02 | Horse sarcoid | Sarcoma | IC50=1.4 ± 0.1 µM | MTT assay | 24 h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | LD50=9 µg/ml | MTT assay | 24 h | Patent |
| SW480 | Colon adenocarcinoma | Carcinoma | LD50=23 µg/ml | MTT assay | 24 h | Patent |
| BMKC | Skin carcinoma | Carcinoma | LD50=18 µg/ml | MTT assay | 24 h | Patent |
| NCI-H1299 | Lung large cell carcinoma | Carcinoma | LD50=25 µg/ml | MTT assay | 24 h | Patent |
| PC-3 | Prostate carcinoma | Carcinoma | LD50=13 µg/ml | MTT assay | 24 h | Patent |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | LD50=35 µg/ml | MTT assay | 24 h | Patent |
Hemolytic Activity high haemolytic activity
Normal (non-cancerous) Cytotoxicity APH-R: IC50=0.9±0.1 μM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antimicrobial; Antifungal
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C131H228N38O32
Absent amino acids CDEFHMNY
Common amino acids L
Mass 329361
Pl 12.55
Basic residues 5
Acidic residues 0
Hydrophobic residues 12
Net charge 5
Boman Index -1482
Hydrophobicity 27.31
Aliphatic Index 135
Half Life
Mammalian: 1.1 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 5500
Absorbance 280nm 220
Polar residues 6
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 23893605
Title Design of NK-2-derived peptides with improved activity against equine sarcoid cells
Doi 10.1002/psc.2540
Year 2013
Patent
Patent ID US2005/0209157A1
Patent Title Short bioactive peptides and methods for their use
Other Iinformation Patent Application; Family: 1s / 24ex; Family Jurisdictions: US; Legal Status: Discontinued; Application No: 13618605; Filed: May 24, 2005; Published: Sep 22, 2005; Earliest Priority: Mar 28, 2001
Other Published ID Not available
External Code
DBAASP ID DBAASPR_806
This work is licensed under a Creative Commons Attribution 4.0 International License.