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Cyclotide mela-1
General Information
DRACP ID DRACP00571
Peptide Name Cyclotide mela-1
Sequence GKYTCGETCFKGKCYTPGCTCSYPICKKD
Sequence Length 29
UniProt ID Not available
PubChem CID Not available
Origin Melicytus latifolius
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | CC50=9.83±0.78µM | Resazurin assay | 24h | 1 |
| MM96L | Melanoma | Carcinoma | CC50=2.09±0.18µM | Resazurin assay | 24h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis>64µM
Normal (non-cancerous) Cytotoxicity HFF-1: CC50=3.07±0.15µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asp29
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C136H210N34O42S6
Absent amino acids AHLMNQRVW
Common amino acids C
Mass 368534
Pl 8.37
Basic residues 5
Acidic residues 2
Hydrophobic residues 2
Net charge 3
Boman Index -3804
Hydrophobicity -56.9
Aliphatic Index 13.45
Half Life
Mammalian: 30 hour
Yeast: >20 hour
E.coli: >10 hour
Extinction Coefficient cystines 4845
Absorbance 280nm 173.04
Polar residues 18
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 26322745
Title Lysine-rich Cyclotides: A New Subclass of Circular Knotted Proteins from Violaceae
Doi 10.1021/acschembio.5b00454
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.