Cyclotide mech-3

General Information


DRACP ID  DRACP00575

Peptide Name   Cyclotide mech-3

Sequence  GLPTCGETCTLGKCNTPKCTCNWPICYKN

Sequence Length  29

UniProt ID  Not available

PubChem CID  Not available

Origin  Melicytus chathamicus

Type  Native peptide

Classification

  

Active ACP



Activity Information


Cell Line Disease Cancer Classified Activity Assay Testing Time Literature
HeLa Human papillomavirus-related endocervical adenocarcinoma Carcinoma CC50=36.92±3.25µM Resazurin assay 24h 1
MM96L Melanoma Carcinoma CC50=21.94±1.90µM Resazurin assay 24h 1

Hemolytic Activity  Human erythrocytes: 50% Hemolysis>64µM

Normal (non-cancerous) Cytotoxicity  HFF-1: CC50=12.45±0.99µM

Target  Not available

Affinity  Not available

Mechanism  Not available

Nature  Anticancer



Structure Information


PDB ID  Not available

Predicted Structure  DRACP00575

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asn29

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C132H210N36O41S6

Absent amino acids  ADFHMQRSV

Common amino acids  C

Mass  364932

Pl  8.11

Basic residues  3

Acidic residues  1

Hydrophobic residues  4

Net charge  2

Boman Index  -2878

Hydrophobicity  -35.52

Aliphatic Index  40.34

Half Life 
  Mammalian: 30 hour
  Yeast: >20 hour
  E.coli: >10 hour

Extinction Coefficient cystines  7365

Absorbance 280nm  263.04

Polar residues  18

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 26322745

Title  Lysine-rich Cyclotides: A New Subclass of Circular Knotted Proteins from Violaceae

Doi 10.1021/acschembio.5b00454

Year  2015

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DRACP is developed by Dr.Zheng's team.