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Ctn[1-14]
General Information
DRACP ID DRACP00581
Peptide Name Ctn[1-14]
Sequence KRFKKFFKKVKKSV
Sequence Length 14
UniProt ID Not available
PubChem CID Not available
Origin Rattlesnake Venom Cathelicidin
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| HeLa S3 | Breast cancer | Carcinoma | IC50=1 µM | Resazurin assay | 4h | 1 |
| U-937 | Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia | Leukemia | IC50=6.25 µM | Resazurin assay | 4h | 1 |
| THP-1 | Childhood acute monocytic leukemia; Acute monoblastic/monocytic leukemia | Leukemia | IC50=25 µM | Resazurin assay | 4h | 1 |
Hemolytic Activity Human erythrocytes: nonhemolytic up to 400 μM, and their equimolar mixture at that concentration caused only 7% hemolysis
Normal (non-cancerous) Cytotoxicity Human Splenic Fibroblasts (HSF): IC50=12.78±0.33 µg/mL; Endometrial epithelial cells(HEEC): IC50=2.66±0.34 µg/mL; HPDE6c7 Human Pancreatic Duct Epithelial Cell: IC50=3.45±0.20 µg/mL; Normal Human Hepatic Cell (Lo-2): IC50=2.91±0.07 µg/mL; NCM460: IC50=3.04±0.10 µg/mL; CCD-19Lu: IC50=4.76±0.03 µg/mL
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antitumor; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C88H148N24O16
Absent amino acids ACDEGHILMNPQTWY
Common amino acids K
Mass 203033
Pl 12.14
Basic residues 8
Acidic residues 0
Hydrophobic residues 5
Net charge 8
Boman Index -4015
Hydrophobicity -112.86
Aliphatic Index 41.43
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 0
Absorbance 280nm 0
Polar residues 1
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 26465972
Title Structural Dissection of Crotalicidin, a Rattlesnake Venom Cathelicidin, Retrieves a Fragment with Antimicrobial and Antitumor Activity
Doi 10.1021/acs.jmedchem.5b01142
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.