This work is licensed under a Creative Commons Attribution 4.0 International License.
Flower-specific defensin / NaD1
General Information
DRACP ID DRACP00612
Peptide Name Flower-specific defensin / NaD1
Sequence RECKTESNTFPGICITKPPCRKACISEKFTDGHCSKILRRCLCTKPC
Sequence Length 47
UniProt ID Not available
PubChem CID Not available
Origin Nicotiana alata
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| PC-3 | Prostate carcinoma | Carcinoma | 100% Killing=25 µM | MTT assay | 48h | 1 |
| U-937 | Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia | Leukemia | 100% Killing=25 µM | MTT assay | 48h | 1 |
Hemolytic Activity Human erythrocytes: 1.5% Hemolysis=50 µM
Normal (non-cancerous) Cytotoxicity Human dermal fibroblasts: 100% Killing=25 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial; Antifungal
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys3<--->Cys47; Cys14<--->Cys34; Cys20<--->Cys41; Cys24<--->Cys43
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C223H374N68O65S8
Absent amino acids MQVWY
Common amino acids C
Mass 612499
Pl 8.8
Basic residues 11
Acidic residues 4
Hydrophobic residues 9
Net charge 7
Boman Index -10707
Hydrophobicity -47.23
Aliphatic Index 51.91
Half Life
Mammalian: 1 hour
Yeast: 2 min
E.coli: 2 min
Extinction Coefficient cystines 500
Absorbance 280nm 10.87
Polar residues 19
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 27503651
Title Nicotiana alata Defensin Chimeras Reveal Differences in the Mechanism of Fungal and Tumor Cell Killing and an Enhanced Antifungal Variant
Year 2016
Literature 2
Pubmed ID 31031739
Title Salt-Tolerant Antifungal and Antibacterial Activities of the Corn Defensin ZmD32
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.