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BLP-7
General Information
DRACP ID DRACP00723
Peptide Name BLP-7
Sequence GIGGALLSAGKSALKGLAKGLAEHFAN
Sequence Length 27
UniProt ID Q9DET7 Q58T59 Q58T89 A0A219CM62
PubChem CID Not available
Origin skin secretion of Oriental fire-bellied toad, Bombina orientalis
Type Synthetic peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| SK-HEP-1 | Liver and intrahepatic bile duct epithelial neoplasm | Carcinoma | IC50=0.61 μM | MTT assay | 48h | 1 |
| Hep-G2 | Hepatoblastoma; Hepatoblastoma | Blastoma | IC50=2.83 μM | MTT assay | 48h | 1 |
| Huh-7 | Adult hepatocellular carcinoma; Adult hepatocellular carcinoma | Carcinoma | IC50=3.87 μM | MTT assay | 48h | 1 |
Hemolytic Activity Human erythrocytes: BLP-7 exhibited rather low cytotoxicity on human erythrocytes at concentration of 50.2 μm (128 mg/ml), and almost no haemolytic activity was detected around its range of MIC values.
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C114H191N33O33
Absent amino acids CDMPQRTVWY
Common amino acids AG
Mass 301595
Pl 10.51
Basic residues 4
Acidic residues 1
Hydrophobic residues 13
Net charge 3
Boman Index 744
Hydrophobicity 41.48
Aliphatic Index 108.89
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 0
Absorbance 280nm 0
Polar residues 9
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28636781
Title Discovery of two bombinin peptides with antimicrobial and anticancer activities from the skin secretion of Oriental fire-bellied toad, Bombina orientalis
Year 2018
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.