I-5

General Information


DRACP ID  DRACP00739

Peptide Name   I-5

Sequence  VRRFRRFFRRLRRSVL

Sequence Length  16

UniProt ID  Not available

PubChem CID  Not available

Origin  cationic host defense peptides

Type  Synthetic peptide

Classification

  

Active ACP



Activity Information


Cell Line Disease Cancer Classified Activity Assay Testing Time Literature
MCF-7 Invasive breast carcinoma of no special type Carcinoma IC50=7.5±0.9 μM MTT assay 48h 1
MCF-7/Taxol Invasive breast carcinoma of no special type Carcinoma IC50=16.8±0.8 μM MTT assay 48h 1
K562 Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia Leukemia IC50=20.4±0.7 μM MTT assay 48h 1
K562/ADM Blast phase chronic myelogenous leukemia; Chronic myeloid leukemia Leukemia IC50=10.5±0.7 μM MTT assay 48h 1
HeLa Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=3.3±1.1 μM MTT assay 48h 1

Hemolytic Activity  Rabbit red blood cells: modest hemolytic activity(as show in Fig.2)

Normal (non-cancerous) Cytotoxicity  HUVEC: IC50=75.84±6.8 μM

Target  Not available

Affinity  Not available

Mechanism  Not available

Nature  Anticancer; Antitumor



Structure Information


PDB ID  Not available

Predicted Structure  DRACP00739

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C100H170N40O18

Absent amino acids  ACDEGHIKMNPQTWY

Common amino acids  R

Mass  248840

Pl  13.35

Basic residues  8

Acidic residues  0

Hydrophobic residues  7

Net charge  8

Boman Index  -9590

Hydrophobicity  -77.5

Aliphatic Index  85

Half Life 
  Mammalian: 100 hour
  Yeast: >20 hour
  E.coli: >10 hour

Extinction Coefficient cystines  0

Absorbance 280nm  0

Polar residues  1

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 28664269

Title  Pro-apoptotic cationic host defense peptides rich in lysine or arginine to reverse drug resistance by disrupting tumor cell membrane

Doi 10.1007/s00726-017-2453-y

Year  2017

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DRACP is developed by Dr.Zheng's team.