Peptide Libraray

Brevinin-1ITa

General Information

DRACP ID DRACP00753

Peptide Name Brevinin-1ITa

Sequence IVPFLLGMVPKLVCLITKKC

Sequence Length 20

UniProt ID C0HL47

PubChem CID Not available

Origin skin secretions of the Italian stream frog Rana italica (Ranidae)

Type Native peptide

Classification

Active ACP

Activity Information

Cell Line	Disease	Cancer Classified	Activity	Assay	Testing Time	Literature
MDA-MB-231	Breast adenocarcinoma	Carcinoma	LC50=8 μM	CellTiter-Glo Lumines- cent Cell Viability assay	24h	1
A549	Lung adenocarcinoma	Carcinoma	LC50=18 μM	CellTiter-Glo Lumines- cent Cell Viability assay	24h	1
HT-29	Colon adenocarcinoma	Carcinoma	LC50=18 μM	CellTiter-Glo Lumines- cent Cell Viability assay	24h	1

Hemolytic Activity mouse erythrocytes: LC50=7 μM

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Not available

Nature Anticancer; Antitumor; Antimicrobial

Structure Information

PDB ID Not available

Predicted Structure DRACP00753

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C105H183N23O22S3

Absent amino acids ADEHNQRSWY

Common amino acids L

Mass 255503

Pl 9.67

Basic residues 3

Acidic residues 0

Hydrophobic residues 10

Net charge 3

Boman Index 3125

Hydrophobicity 152.5

Aliphatic Index 160.5

Half Life
Mammalian: 1.1 hour
Yeast: 3 min
E.coli: 2 min

Extinction Coefficient cystines 125

Absorbance 280nm 6.58

Polar residues 4

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 28699258

Title Cytotoxic peptides with insulin-releasing activities from skin secretions of the Italian stream frog Rana italica (Ranidae)

Doi 10.1002/psc.3025

Year 2017

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available