This work is licensed under a Creative Commons Attribution 4.0 International License.
[R4A,R18A]cGm
General Information
DRACP ID DRACP00754
Peptide Name [R4A,R18A]cGm
Sequence GCRALCYKQRCVTYCRGA
Sequence Length 18
UniProt ID Not available
PubChem CID Not available
Origin Redesigned Spider Peptide
Type Synthetic peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| CRL-1739 | Gastric adenocarcinoma | Carcinoma | CC50>64 µM | Resazurin assay | 18-24h | 1 |
| MM96L | Melanoma | Carcinoma | CC50=10.3 ± 1.1 µM | Resazurin assay | 18-24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | CC50=>64 µM | Resazurin assay | 18-24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | CC50=48.4 ± 0.7 µM | Resazurin assay | 18-24h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | Leukemia | CC50=11.5 ± 0.6 µM | Resazurin assay | 18-24h | 1 |
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | Leukemia | CC50=34.1 ± 3.9 µM | Resazurin assay | 18-24h | 1 |
Hemolytic Activity Human red blood cells (hRBCs): hemolysis(%)=39.3±9.3 with 64 μM of peptide
Normal (non-cancerous) Cytotoxicity PBMCs:CC50=30.8±2.5 µM, testing time is 18-24h; HFF-1: CC50=51.2 ± 4.0 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys2<--->Cys15; Cys6<--->Cys11
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C84H139N29O23S4
Absent amino acids DEFHIMNPSW
Common amino acids C
Mass 235487
Pl 9.03
Basic residues 4
Acidic residues 0
Hydrophobic residues 4
Net charge 4
Boman Index -3912
Hydrophobicity -18.89
Aliphatic Index 48.89
Half Life
Mammalian: 20 hour
Yeast: 30 min
E.coli: >10 hour
Extinction Coefficient cystines 3230
Absorbance 280nm 190
Polar residues 9
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28741926
Title Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties
Doi 10.1021/acschembio.7b00459
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.