This work is licensed under a Creative Commons Attribution 4.0 International License.
[DPLP]cGm
General Information
DRACP ID DRACP00758
Peptide Name [DPLP]cGm
Sequence GCRRLCYKQRCVTYCRGpPR
Sequence Length 20
UniProt ID Not available
PubChem CID Not available
Origin Redesigned Spider Peptide
Type Synthetic peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| CRL-1739 | Gastric adenocarcinoma | Carcinoma | CC50=22.5 ± 3.3 µM | Resazurin assay | 18-24h | 1 |
| MM96L | Melanoma | Carcinoma | CC50=3.0 ± 0.1 µM | Resazurin assay | 18-24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | CC50=51.5 ± 3.9 µM | Resazurin assay | 18-24h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | Leukemia | CC50=3.9 ± 0.2 µM | Resazurin assay | 18-24h | 1 |
Hemolytic Activity Human red blood cells (hRBCs): hemolysis(%)=43.2±1.7 with 64 μM of peptide
Normal (non-cancerous) Cytotoxicity PBMCs:CC50=14.1±0.9 µM, testing time is 18-24h; HFF-1: CC50=14.9 ± 0.8 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys2<--->Cys15; Cys6<--->Cys11
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification p=D-Proline
Chiral Mix
Physicochemical Information
Formula C95H158N36O23S4
Absent amino acids ADEFHIMNSW
Common amino acids R
Mass 263984
Pl 10.07
Basic residues 6
Acidic residues 0
Hydrophobic residues 2
Net charge 6
Boman Index -7258
Hydrophobicity -88
Aliphatic Index 34
Half Life
/
Extinction Coefficient cystines 3230
Absorbance 280nm 170
Polar residues 9
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28741926
Title Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties
Doi 10.1021/acschembio.7b00459
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.