cGm

General Information


DRACP ID  DRACP00759

Peptide Name   cGm

Sequence  GCRRLCYKQRCVTYCRGR

Sequence Length  18

UniProt ID  P82358 

PubChem CID  Not available

Origin  Redesigned Spider Peptide

Type  Synthetic peptide

Classification

  

Active ACP



Activity Information


Cell Line Disease Cancer Classified Activity Assay Testing Time Literature
CRL-1739 Gastric adenocarcinoma Carcinoma CC50=39.8 ± 1.0 µM Resazurin assay 18-24h 1
MM96L Melanoma Carcinoma CC50=2.9 ± 0.1 µM Resazurin assay 18-24h 1
HeLa Human papillomavirus-related endocervical adenocarcinoma Carcinoma CC50=64 µM Resazurin assay 18-24h 1
MCF-7 Invasive breast carcinoma of no special type Carcinoma CC50=15.3 ± 1.4 µM Resazurin assay 18-24h 1
K562 Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia Leukemia CC50=2.7 ± 0.1 µM Resazurin assay 18-24h 1
HL-60 Adult acute myeloid leukemia; Acute myeloid leukemia Leukemia CC50=12.7 ± 1.1 µM Resazurin assay 18-24h 1

Hemolytic Activity  Human red blood cells (hRBCs): hemolysis(%)=45.9±9.4 with 64 μM of peptide

Normal (non-cancerous) Cytotoxicity  PBMCs:CC50=10.8±0.8 µM, testing time is 18-24h

Target  Not available

Affinity  Not available

Mechanism  Not available

Nature  Anticancer; Antimicrobial



Structure Information


PDB ID  1KFP 

Predicted Structure  DRACP00759

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys2<--->Cys15; Cys6<--->Cys11

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C90H153N35O23S4

Absent amino acids  ADEFHIMNPSW

Common amino acids  R

Mass  252507

Pl  10.07

Basic residues  6

Acidic residues  0

Hydrophobic residues  2

Net charge  6

Boman Index  -7258

Hydrophobicity  -88.89

Aliphatic Index  37.78

Half Life 
  Mammalian: 30 hour
  Yeast: >20 hour
  E.coli: >10 hour

Extinction Coefficient cystines  3230

Absorbance 280nm  190

Polar residues  9

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 28741926

Title  Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties

Doi 10.1021/acschembio.7b00459

Year  2017

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DRACP is developed by Dr.Zheng's team.