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[C/U]cGm
General Information
DRACP ID DRACP00762
Peptide Name [C/U]cGm
Sequence GURRLUYKQRUVTYURGR
Sequence Length 18
UniProt ID Not available
PubChem CID Not available
Origin Redesigned Spider Peptide
Type Synthetic peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| CRL-1739 | Gastric adenocarcinoma | Carcinoma | CC50=51.0 ± 4.6 µM | Resazurin assay | 18-24h | 1 |
| MM96L | Melanoma | Carcinoma | CC50=4.6 ± 0.2 µM | Resazurin assay | 18-24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | CC50>64 µM | Resazurin assay | 18-24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | CC50=37.5 ± 3.3 µM | Resazurin assay | 18-24h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | Leukemia | CC50=1.4 ± 0.2 µM | Resazurin assay | 18-24h | 1 |
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | Leukemia | CC50=38.5 ± 4.8 µM | Resazurin assay | 18-24h | 1 |
Hemolytic Activity Human red blood cells (hRBCs): hemolysis(%)=32.8±0.5 with 64 μM of peptide
Normal (non-cancerous) Cytotoxicity PBMCs:CC50=15.5±0.8 µM, testing time is 18-24h; HFF-1: CC50=51.0 ± 4.6 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond SelenoCys2<--->SelenoCys; SelenoCys<--->SelenoCys11
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification U=SelenoCys
Chiral L
Physicochemical Information
Formula C78H125N31O15
Absent amino acids ACDEFHIMNPSW
Common amino acids R
Mass 204043
Pl 12.34
Basic residues 6
Acidic residues 0
Hydrophobic residues 2
Net charge 6
Boman Index -7770
Hydrophobicity -144.44
Aliphatic Index 37.78
Half Life
/
Extinction Coefficient cystines 2980
Absorbance 280nm 175.29
Polar residues 5
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28741926
Title Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties
Doi 10.1021/acschembio.7b00459
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.