[G1K,K8R]cGm
General Information
DRACP ID DRACP00764
Peptide Name [G1K,K8R]cGm
Sequence KCRRLCYRQRCVTYCRGR
Sequence Length 18
UniProt ID Not available
PubChem CID Not available
Origin Redesigned Spider Peptide
Type Synthetic peptide
Classification
Active ACP
Activity Information
Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
---|---|---|---|---|---|---|
CRL-1739 | Gastric adenocarcinoma | Carcinoma | CC50=19.5 ± 0.5 µM | Resazurin assay | 18-24h | 1 |
MM96L | Melanoma | Carcinoma | CC50=1.7 ± 0.1 µM | Resazurin assay | 18-24h | 1 |
HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | CC50=44.2 ± 2.2 µM | Resazurin assay | 18-24h | 1 |
MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | CC50=6.7 ± 0.7 µM | Resazurin assay | 18-24h | 1 |
K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | Leukemia | CC50=2.1 ± 0.2 µM | Resazurin assay | 18-24h | 1 |
HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | Leukemia | CC50=9.0 ± 1.1 µM | Resazurin assay | 18-24h | 1 |
Hemolytic Activity Human red blood cells (hRBCs): hemolysis(%)=45.1±11.8 with 64 μM of peptide
Normal (non-cancerous) Cytotoxicity PBMCs:CC50=5.1±0.3 µM , testing time is 18-24h; HFF-1: CC50=9.0 ± 0.5 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys2<--->Cys15; Cys6<--->Cys11
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C94H162N38O23S4
Absent amino acids ADEFHIMNPSW
Common amino acids R
Mass 262420
Pl 10.64
Basic residues 7
Acidic residues 0
Hydrophobic residues 2
Net charge 7
Boman Index -8844
Hydrophobicity -111.67
Aliphatic Index 37.78
Half Life
Mammalian: 30 hour
Yeast: >20 hour
E.coli: >10 hour
Extinction Coefficient cystines 3230
Absorbance 280nm 190
Polar residues 8
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28741926
Title Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties
Doi 10.1021/acschembio.7b00459
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available