This work is licensed under a Creative Commons Attribution 4.0 International License.
[G1K,L5Y,K8R]cGm
General Information
DRACP ID DRACP00765
Peptide Name [G1K,L5Y,K8R]cGm
Sequence KCRRYCYRQRCVTYCRGR
Sequence Length 18
UniProt ID Not available
PubChem CID Not available
Origin Redesigned Spider Peptide
Type Synthetic peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| CRL-1739 | Gastric adenocarcinoma | Carcinoma | CC50=26.5 ± 2.4 µM | Resazurin assay | 18-24h | 1 |
| MM96L | Melanoma | Carcinoma | CC50=2.0 ± 0.1 µM | Resazurin assay | 18-24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | CC50=20.9 ± 1.4 µM | Resazurin assay | 18-24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | CC50=15.2 ± 1.9 µM | Resazurin assay | 18-24h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | Leukemia | CC50=1.3 ± 0.1 µM | Resazurin assay | 18-24h | 1 |
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | Leukemia | CC50=15.7 ± 1.1 µM | Resazurin assay | 18-24h | 1 |
Hemolytic Activity Human red blood cells (hRBCs): hemolysis(%)=26.3±5.7 with 64 μM of peptide
Normal (non-cancerous) Cytotoxicity PBMCs:CC50=10.4±1.0µM, testing time is 18-24h; HFF-1: CC50=14.7 ± 1.5 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys2<--->Cys15; Cys6<--->Cys11
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C97H160N38O24S4
Absent amino acids ADEFHILMNPSW
Common amino acids R
Mass 267421
Pl 10.28
Basic residues 7
Acidic residues 0
Hydrophobic residues 1
Net charge 7
Boman Index -9350
Hydrophobicity -140
Aliphatic Index 16.11
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 4720
Absorbance 280nm 277.65
Polar residues 9
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28741926
Title Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties
Doi 10.1021/acschembio.7b00459
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.