This work is licensed under a Creative Commons Attribution 4.0 International License.
[C/U,G1K,L5Y,K8R]cGm
General Information
DRACP ID DRACP00766
Peptide Name [C/U,G1K,L5Y,K8R]cGm
Sequence KURRYUYRQRUVTYURGR
Sequence Length 18
UniProt ID Not available
PubChem CID Not available
Origin Redesigned Spider Peptide
Type Synthetic peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| CRL-1739 | Gastric adenocarcinoma | Carcinoma | CC50=46.2 ± 7.6 µM | Resazurin assay | 18-24h | 1 |
| MM96L | Melanoma | Carcinoma | CC50=2.3 ± 0.2 µM | Resazurin assay | 18-24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | CC50=36.2 ± 2.8 µM | Resazurin assay | 18-24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | CC50=29.0 ± 3.7 µM | Resazurin assay | 18-24h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | Leukemia | CC50=6.4 ± 0.6 µM | Resazurin assay | 18-24h | 1 |
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | Leukemia | CC50=33.3 ± 7.4 µM | Resazurin assay | 18-24h | 1 |
Hemolytic Activity Human red blood cells (hRBCs): hemolysis(%)=20.9±3.1 with 64 μM of peptide
Normal (non-cancerous) Cytotoxicity PBMCs:CC50=9.5±0.5 µM, testing time is 18-24h; HFF-1: CC50=30.8 ± 2.0 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond SelenoCys2<--->SelenoCys15; SelenoCys6<--->SelenoCys11
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification U=SelenoCys
Chiral L
Physicochemical Information
Formula C85H132N34O16
Absent amino acids ACDEFHILMNPSW
Common amino acids R
Mass 218957
Pl 12.22
Basic residues 7
Acidic residues 0
Hydrophobic residues 1
Net charge 7
Boman Index -9862
Hydrophobicity -195.56
Aliphatic Index 16.11
Half Life
/
Extinction Coefficient cystines 4470
Absorbance 280nm 262.94
Polar residues 5
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28741926
Title Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties
Doi 10.1021/acschembio.7b00459
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.