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Gm
General Information
DRACP ID DRACP00768
Peptide Name Gm
Sequence XCRRLCYKQRCVTYCRGR
Sequence Length 18
UniProt ID Not available
PubChem CID Not available
Origin Redesigned Spider Peptide
Type Synthetic peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| CRL-1739 | Gastric adenocarcinoma | Carcinoma | CC50=67.0 ± 5.0 µM | Resazurin assay | 18-24h | 1 |
| MM96L | Melanoma | Carcinoma | CC50=3.7 ± 0.2 µM | Resazurin assay | 18-24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | CC50=54.1 ± 5.0 µM | Resazurin assay | 18-24h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | Leukemia | CC50=3.8 ± 0.3 µM | Resazurin assay | 18-24h | 1 |
Hemolytic Activity Human red blood cells (hRBCs): hemolysis(%)=30.7±8.1 with 64 μM of peptide
Normal (non-cancerous) Cytotoxicity PBMCs: CC50=13.4±1.8 µM, testing time is 18-24h; HFF-1: CC50=49.9 ± 16.6 µM, testing time is 18-24h
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys2<--->Cys15; Cys6<--->Cys11
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification X=N-terminal pyroglutamic acid
Chiral L
Physicochemical Information
Formula C88H148N34O21S4
Absent amino acids ADEFHIMNPSW
Common amino acids R
Mass 256000
Pl 10.07
Basic residues 6
Acidic residues 0
Hydrophobic residues 2
Net charge 6
Boman Index -7352
Hydrophobicity -86.67
Aliphatic Index 37.78
Half Life
/
Extinction Coefficient cystines 3230
Absorbance 280nm 190
Polar residues 8
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28741926
Title Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties
Doi 10.1021/acschembio.7b00459
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_814
This work is licensed under a Creative Commons Attribution 4.0 International License.