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Phylloseptin-PHa
General Information
DRACP ID DRACP00776
Peptide Name Phylloseptin-PHa
Sequence FLSLIPAAISAVSALANHF
Sequence Length 19
UniProt ID Not available
PubChem CID Not available
Origin Pithecopus hypochondrialis
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| MDA-MB-435S | Amelanotic melanoma | Carcinoma | LD50=28.48µM | MTT assay | 24h | 1 |
| NCI-H157 | Lung squamous cell carcinoma | Carcinoma | LD50=37.61µM | MTT assay | 24h | 1 |
| PC-3 | Prostate carcinoma | Carcinoma | LD50=37.96µM | MTT assay | 24h | 1 |
| HCT 116 | Colon carcinoma | Carcinoma | LD50=36.64µM | MTT assay | 24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | LD50=24.05µM | MTT assay | 24h | 1 |
| U-251MG | Astrocytoma | Carcinoma | LD50=34.67µM | MTT assay | 24h | 1 |
Hemolytic Activity Horse erythrocytes: 10% Hemolysis=76.5µM; 50% Hemolysis=109.5µM; 50% Hemolysis=52.17µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial; Antifungal
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C92H144N22O24
Absent amino acids CDEGKMQRTWY
Common amino acids A
Mass 226355
Pl 7.55
Basic residues 1
Acidic residues 0
Hydrophobic residues 13
Net charge 1
Boman Index 2215
Hydrophobicity 150
Aliphatic Index 144.21
Half Life
Mammalian: 20 hour
Yeast: 30 min
E.coli: >10 hour
Extinction Coefficient cystines 0
Absorbance 280nm 0
Polar residues 4
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 28850103
Title Discovery of Phylloseptins that Defense against Gram-Positive Bacteria and Inhibit the Proliferation of the Non-Small Cell Lung Cancer Cell Line, from the Skin Secretions of Phyllomedusa Frogs
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.