This work is licensed under a Creative Commons Attribution 4.0 International License.
HD5
General Information
DRACP ID DRACP00805
Peptide Name HD5
Sequence ATCYCRTGRCATRESLSGVCEISGRLYRLCCR
Sequence Length 32
UniProt ID Not available
PubChem CID Not available
Origin Homo sapiens
Type Native peptide
Classification
Active ACP Membrane-targeted
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| JLTRG (Jurkat LTR-GFP Cells) | Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia | Leukemia | Not active up to 50 µg/ml | CCK-8 assay | 36h | 1 |
Hemolytic Activity Human erythrocytes: NA; Mouse erythrocytes: 20% Hemolysis=25 µg/ml; 33% Hemolysis=50 µg/ml; 40% Hemolysis=100 µg/ml
Normal (non-cancerous) Cytotoxicity HaCat: 10% Killing=150 µg/ml; ARPE-19: 50% Cytotoxicity=24 µM; CaSki: Not active up to 50 µg/ml
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial; Antifungal; Antiviral
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C144H244N50O45S6
Absent amino acids DFHKMNPQW
Common amino acids CR
Mass 414131
Pl 8.62
Basic residues 6
Acidic residues 2
Hydrophobic residues 7
Net charge 4
Boman Index -8349
Hydrophobicity -11.25
Aliphatic Index 64.06
Half Life
Mammalian: 1.1 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 3355
Absorbance 280nm 108.23
Polar residues 17
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 23269800
Title Enhancement of antiviral activity of human alpha-defensin 5 against herpes simplex virus 2 by arginine mutagenesis at adaptive evolution sites
Year 2013
Literature 2
Pubmed ID 29158275
Title A Simplified Derivative of Human Defensin 5 with Potent and Efficient Activity against Multidrug-Resistant Acinetobacter baumannii
Year 2018
Literature 3
Pubmed ID 32209394
Title The human α-defensin-derived peptide HD5(1-9) inhibits cellular attachment and entry of human cytomegalovirus
Doi 10.1016/j.antiviral.2020.104779
Year 2020
Literature 4
Pubmed ID 26206286
Title Antimicrobial activity of human α-defensin 5 and its linear analogs: N-terminal fatty acylation results in enhanced antimicrobial activity of the linear analogs
Doi 10.1016/j.peptides.2015.07.009
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.