This work is licensed under a Creative Commons Attribution 4.0 International License.
Brevinin-1DYa
General Information
DRACP ID DRACP00812
Peptide Name Brevinin-1DYa
Sequence FLSLALAALPKFLCLVFKKC
Sequence Length 20
UniProt ID Not available
PubChem CID Not available
Origin Rana dybowskii; Rana huanrenensis; Rana amurensis
Type Native peptide
Classification
Active ACP Membrane-targeted
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| NCI-H157 | Lung squamous cell carcinoma | Carcinoma | IC50=10.6 µM | MTT assay | Not available | 1 |
| PC-3 | Prostate carcinoma | Carcinoma | IC50=7.0 µM | MTT assay | Not available | 1 |
| U-251MG | Astrocytoma | Carcinoma | IC50=4.1 µM | MTT assay | Not available | 1 |
| MDA-MB-435S | Amelanotic melanoma | Carcinoma | IC50=10.3 µM | MTT assay | Not available | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | IC50=14.6 µM | MTT assay | Not available | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis<5 µM; Horse erythrocytes: 50% Hemolysis=17.0 µM
Normal (non-cancerous) Cytotoxicity HMEC-1: IC50=35.9 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antibacterial; Antifungal
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys14<--->Cys20
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C109H177N23O22S2
Absent amino acids DEGHIMNQRTWY
Common amino acids L
Mass 256497
Pl 9.67
Basic residues 3
Acidic residues 0
Hydrophobic residues 13
Net charge 3
Boman Index 3044
Hydrophobicity 158.5
Aliphatic Index 146.5
Half Life
Mammalian: 1.4 hour
Yeast: 3 min
E.coli: >10 hour
Extinction Coefficient cystines 125
Absorbance 280nm 6.58
Polar residues 3
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 29366784
Title Identification of novel Amurin-2 variants from the skin secretion of Rana amurensis, and the design of cationicity-enhanced analogues
Doi 10.1016/j.bbrc.2018.01.124
Year 2018
Literature 2
Pubmed ID 17688900
Title Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii
Doi 10.1016/j.toxicon.2007.06.023
Year 2007
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.