Peptide Libraray

LyeTxI-b

General Information

DRACP ID DRACP00850

Peptide Name LyeTxI-b

Sequence IWLTALKFLGKNLGKLAKQQLAKL

Sequence Length 24

UniProt ID Not available

PubChem CID Not available

Origin Lycosa erythrognatha spider venom

Type Native peptide

Classification

Active ACP

Activity Information

Cell Line	Disease	Cancer Classified	Activity	Assay	Testing Time	Literature
U-87MG ATCC	Glioblastoma	Blastoma	IC50=29.20±7.96 μM	MTT assay	48h	1
U-373MG ATCC	Astrocytoma	Carcinoma	IC50=20.94±5.18 μM	MTT assay	48h	1
SH-SY5Y	Neuroblastoma	Blastoma	IC50=93.80±2.17 μM	MTT assay	48h	1

Hemolytic Activity hRBC(human blood red cells): IC50=155±13.04 μM (testing time is 48 h)

Normal (non-cancerous) Cytotoxicity Vero(African green monkey kidney): IC50≥100 μM (testing time is 48 h); PBMC(Peripheral Blood Mononuclear Cells): IC50=4.16±1.94 μM (testing time is 48 h); GM00637: IC50 ≥ 100 μM

Target Not available

Affinity Not available

Mechanism Not available

Nature Anticancer; Antitumor

Structure Information

PDB ID Not available

Predicted Structure DRACP00850

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C129H219N33O29

Absent amino acids CDEHMPRSVY

Common amino acids L

Mass 310683

Pl 11.4

Basic residues 5

Acidic residues 0

Hydrophobic residues 13

Net charge 5

Boman Index 394

Hydrophobicity 28.75

Aliphatic Index 142.5

Half Life
Mammalian: >20 hour
Yeast: >20 hour
E.coli: ?

Extinction Coefficient cystines 5500

Absorbance 280nm 239.13

Polar residues 4

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 30449002

Title The synthetic peptide LyeTxI-b derived from Lycosa erythrognatha spider venom is cytotoxic to U-87 MG glioblastoma cells

Doi 10.1007/s00726-018-2678-4

Year 2019

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available