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TP III
General Information
DRACP ID DRACP00852
Peptide Name TP III
Sequence KWCFRVCYRGICYRKCR
Sequence Length 17
UniProt ID P18252
PubChem CID Not available
Origin horseshoe crab Limulus polyphemus
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | Leukemia | IC50=5.0±0.4 μM | MTT assay | 48h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50=13.7±1.3 μM | MTT assay | 48h | 1 |
| SK-BR-3 | Breast adenocarcinoma | Carcinoma | IC50=27.5±0.8 μM | MTT assay | 48h | 1 |
| A549 | Lung adenocarcinoma | Carcinoma | IC50=15.3±1.0 μM | MTT assay | 48h | 1 |
Hemolytic Activity Human red blood cells (hBRC): TP I lysed 20%, at a maximal concentration of 100 µM
Normal (non-cancerous) Cytotoxicity HEK 293T: IC50=19.7±1.9 μM; HEF:IC50=14.1±1.2 μM; NHA: IC50=14.1±1.2 μM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C99H154N32O20S4
Absent amino acids ADEHLMNPQST
Common amino acids CR
Mass 252632
Pl 9.99
Basic residues 6
Acidic residues 0
Hydrophobic residues 4
Net charge 6
Boman Index -5073
Hydrophobicity -48.24
Aliphatic Index 40
Half Life
Mammalian: 3.5 hour
Yeast: 10 min
E.coli: >10 hour
Extinction Coefficient cystines 8730
Absorbance 280nm 545.63
Polar residues 7
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 30486233
Title Cytotoxic Potential of the Novel Horseshoe Crab Peptide Polyphemusin III
Year 2018
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.