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PM II
General Information
DRACP ID DRACP00855
Peptide Name PM II
Sequence RRWCFRVCYKGFCYRKCR
Sequence Length 18
UniProt ID P14216
PubChem CID Not available
Origin horseshoe crab Limulus polyphemus
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | Leukemia | IC50=7.2±0.5 μM | MTT assay | 48h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50=16.4±1.3 μM | MTT assay | 48h | 1 |
| SK-BR-3 | Breast adenocarcinoma | Carcinoma | IC50=17.3±0.6 μM | MTT assay | 48h | 1 |
| A549 | Lung adenocarcinoma | Carcinoma | IC50=10.8±1.9 μM | MTT assay | 48h | 1 |
Hemolytic Activity Human red blood cells (hBRC): only ~30%, even at the maximal concentration of 100 µM
Normal (non-cancerous) Cytotoxicity HEK 293T: IC50=11.3±1.6 μM; HEF: IC50=9.7±0.2 μM; NHA: IC50=18.3±1.4 μM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C108H164N36O21S4
Absent amino acids ADEHILMNPQST
Common amino acids R
Mass 273435
Pl 10.46
Basic residues 7
Acidic residues 0
Hydrophobic residues 4
Net charge 7
Boman Index -6759
Hydrophobicity -80
Aliphatic Index 16.11
Half Life
Mammalian: 1 hour
Yeast: 2 min
E.coli: 2 min
Extinction Coefficient cystines 8730
Absorbance 280nm 513.53
Polar residues 7
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 30486233
Title Cytotoxic Potential of the Novel Horseshoe Crab Peptide Polyphemusin III
Year 2018
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.