PM I

General Information


DRACP ID  DRACP00856

Peptide Name   PM I

Sequence  RRWCFRVCYRGFCYRKCR

Sequence Length  18

UniProt ID  P14215 

PubChem CID  Not available

Origin  horseshoe crab Limulus polyphemus

Type  Native peptide

Classification

  

Active ACP



Activity Information


Cell Line Disease Cancer Classified Activity Assay Testing Time Literature
HL-60 Adult acute myeloid leukemia; Acute myeloid leukemia Leukemia IC50=7.2±0.5 μM MTT assay 48h 1
HeLa Human papillomavirus-related endocervical adenocarcinoma Carcinoma IC50=12.5±0.9 μM MTT assay 48h 1
SK-BR-3 Breast adenocarcinoma Carcinoma IC50=16.0±0.6 μM MTT assay 48h 1
A549 Lung adenocarcinoma Carcinoma IC50=8.8±1.3 μM MTT assay 48h 1

Hemolytic Activity  Human red blood cells (hBRC): only ~45%, even at the maximal concentration of 100 µM

Normal (non-cancerous) Cytotoxicity  HEK 293T: IC50=9.4±1.5 μM; HEF: IC50=8.3±0.7 μM; NHA: IC50=14.5±1.6 μM

Target  Not available

Affinity  Not available

Mechanism  Not available

Nature  Anticancer



Structure Information


PDB ID  1RKK 

Predicted Structure  DRACP00856

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C108H164N38O21S4

Absent amino acids  ADEHILMNPQST

Common amino acids  R

Mass  276236

Pl  10.64

Basic residues  7

Acidic residues  0

Hydrophobic residues  4

Net charge  7

Boman Index  -7696

Hydrophobicity  -83.33

Aliphatic Index  16.11

Half Life 
  Mammalian: 1 hour
  Yeast: 2 min
  E.coli: 2 min

Extinction Coefficient cystines  8730

Absorbance 280nm  513.53

Polar residues  7

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 30486233

Title  Cytotoxic Potential of the Novel Horseshoe Crab Peptide Polyphemusin III

Doi 10.3390/md16120466

Year  2018

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DRACP is developed by Dr.Zheng's team.