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ChMAP-28
General Information
DRACP ID DRACP00863
Peptide Name ChMAP-28
Sequence GRFKRFRKKLKRLWHKVGPFVGPILHY
Sequence Length 27
UniProt ID Not available
PubChem CID Not available
Origin Goat Antimicrobial
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | Leukemia | IC50=3.39±0.15 μM | MTT assay | 48h | 1 |
| SK-BR-3 | Breast adenocarcinoma | Carcinoma | IC50=5.63±1.05 μM | MTT assay | 48h | 1 |
| A431 | Skin squamous cell carcinoma | Carcinoma | IC50=6.49±0.09 μM | MTT assay | 48h | 1 |
| B16-F1 | Mouse melanoma | Carcinoma | IC50=4.82±1.01 μM | MTT assay | 48h | 1 |
Hemolytic Activity Human erythrocytes: ChMAP-28 has a low hemolytic activity (hemolysis less than 10%) at a concentration range up to 10 μM.
Normal (non-cancerous) Cytotoxicity HEK293T: IC50=5.09±0.40 μM; HEF: IC50=8.95±2.68 μM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C163H255N49O29
Absent amino acids ACDEMNQST
Common amino acids K
Mass 382905
Pl 12.54
Basic residues 11
Acidic residues 0
Hydrophobic residues 10
Net charge 11
Boman Index -5504
Hydrophobicity -65.93
Aliphatic Index 79.26
Half Life
Mammalian: 1.1 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 6990
Absorbance 280nm 268.85
Polar residues 4
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 30622471
Title Anticancer Activity of the Goat Antimicrobial Peptide ChMAP-28
Year 2018
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.