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TI
General Information
DRACP ID DRACP00916
Peptide Name TI
Sequence KWCFRVCYRGICYRRCR
Sequence Length 17
PubChem CID Not available
Origin host defense peptide from the horseshoe crab Tachypleus tridentatus
Type Synthetic peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| MM96L | Melanoma | Carcinoma | CC50=1.5±0.1 µM | Resazurin assay | 24h | 1 |
| HT-144 | Melanoma | Carcinoma | CC50=1.7±0.2 µM | Resazurin assay | 24h | 1 |
| WM164 | Cutaneous melanoma | Carcinoma | CC50=2.5±0.1 µM | Resazurin assay | 24h | 1 |
| MDA-MB-435S | Amelanotic melanoma | Carcinoma | CC50=8.0±0.6 µM | Resazurin assay | 24h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | Leukemia | CC50=1.7±0.1 µM | Resazurin assay | 24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | Carcinoma | CC50=16.0±2.0 µM | Resazurin assay | 24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | CC50=13.1±1.2 µM | Resazurin assay | 24h | 1 |
Hemolytic Activity Human RBCs: CC50=34.9±2.8 µM
Normal (non-cancerous) Cytotoxicity HaCaT: CC50=16.0±1.5 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antitumor
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys3<--->Cys16; Cys7<--->Cys12
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C99H154N34O20S4
Absent amino acids ADEHLMNPQST
Common amino acids R
Mass 255433
Pl 10.07
Basic residues 6
Acidic residues 0
Hydrophobic residues 4
Net charge 6
Boman Index -6010
Hydrophobicity -51.76
Aliphatic Index 40
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 8730
Absorbance 280nm 545.63
Polar residues 7
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 31714739
Title Cyclic Analogues of Horseshoe Crab Peptide Tachyplesin I with Anticancer and Cell Penetrating Properties
Doi 10.1021/acschembio.9b00782
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.