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[G18K]cTI
General Information
DRACP ID DRACP00918
Peptide Name [G18K]cTI
Sequence KWCFRVCYRGICYRRCRK
Sequence Length 18
PubChem CID Not available
Origin host defense peptide from the horseshoe crab Tachypleus tridentatus
Type Synthetic peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| WM164 | Cutaneous melanoma | Carcinoma | CC50=2.7±0.2 µM | Resazurin assay | 24h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | Leukemia | CC50=1.7±0.1 µM | Resazurin assay | 24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | CC50=6.7±1.1 µM | Resazurin assay | 24h | 1 |
| MM96L | Melanoma | Carcinoma | CC50=1.0±0.1 µM | Resazurin assay | 24h | 1 |
| HT-144 | Melanoma | Carcinoma | CC50=3.2±0.1 µM | Resazurin assay | 24h | 1 |
Hemolytic Activity Human RBCs: CC50=6.9±0.3 µM
Normal (non-cancerous) Cytotoxicity HaCaT: CC50>64 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antitumor
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys3<--->Cys16; Cys7<--->Cys12
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C105H166N36O21S4
Absent amino acids ADEHLMNPQST
Common amino acids R
Mass 270034
Pl 10.46
Basic residues 7
Acidic residues 0
Hydrophobic residues 4
Net charge 7
Boman Index -6565
Hydrophobicity -70.56
Aliphatic Index 37.78
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 8730
Absorbance 280nm 513.53
Polar residues 7
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 31714739
Title Cyclic Analogues of Horseshoe Crab Peptide Tachyplesin I with Anticancer and Cell Penetrating Properties
Doi 10.1021/acschembio.9b00782
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.