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[R14S]cTI
General Information
DRACP ID DRACP00920
Peptide Name [R14S]cTI
Sequence KWCFRVCYRGICYSRCRG
Sequence Length 18
UniProt ID Not available
PubChem CID Not available
Origin host defense peptide from the horseshoe crab Tachypleus tridentatus
Type Synthetic peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| WM164 | Cutaneous melanoma | Carcinoma | CC50=2.7±0.1 µM | Resazurin assay | 24h | 1 |
| MDA-MB-435S | Amelanotic melanoma | Carcinoma | CC50=3.5±0.3 µM | Resazurin assay | 24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | CC50=7.5±0.3 µM | Resazurin assay | 24h | 1 |
| MM96L | Melanoma | Carcinoma | CC50=1.7±0.1 µM | Resazurin assay | 24h | 1 |
| HT-144 | Melanoma | Carcinoma | CC50=2.3±0.2 µM | Resazurin assay | 24h | 1 |
Hemolytic Activity Human RBCs: CC50=5.3±0.3 µM
Normal (non-cancerous) Cytotoxicity HaCaT: CC50>64 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antitumor
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys3<--->Cys16; Cys7<--->Cys12
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C98H150N32O22S4
Absent amino acids ADEHLMNPQT
Common amino acids CR
Mass 256011
Pl 9.51
Basic residues 5
Acidic residues 0
Hydrophobic residues 4
Net charge 5
Boman Index -4764
Hydrophobicity -30.56
Aliphatic Index 37.78
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 8730
Absorbance 280nm 513.53
Polar residues 9
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 31714739
Title Cyclic Analogues of Horseshoe Crab Peptide Tachyplesin I with Anticancer and Cell Penetrating Properties
Doi 10.1021/acschembio.9b00782
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.