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Brevinin-1RL1
General Information
DRACP ID DRACP01055
Peptide Name Brevinin-1RL1
Sequence FFPLIAGLAARFLPKIFCSITKRC
Sequence Length 24
UniProt ID Not available
PubChem CID Not available
Origin Frog Skin Secretion
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| HCT 116 | Colon carcinoma | Carcinoma | IC50=5.87±0.15 µM | MTS assay | 48h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | Carcinoma | IC50=5.44±0.33 µM | MTS assay | 48h | 1 |
| SW480 | Colon adenocarcinoma | Carcinoma | IC50=10.37±0.40 µM | MTS assay | 48h | 1 |
| A549 | Lung adenocarcinoma | Carcinoma | IC50=5.81±0.23 µM | MTS assay | 48h | 1 |
| SMMC-7721 | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50=6.87±0.51 µM | MTS assay | 48h | 1 |
| B16-F10 | Mouse melanoma | Carcinoma | IC50=6.65±0.33 µM | MTS assay | 48h | 1 |
Hemolytic Activity Human erythrocytes: Moderate Hemolysis
Normal (non-cancerous) Cytotoxicity NCM460: IC50=16.84±0.56 µM; BEAS-2B: IC50=16.57±0.29 µM; HaCaT: IC50=28.67±0.36 µM
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antitumor; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C130H206N32O27S2
Absent amino acids DEHMNQVWY
Common amino acids F
Mass 312382
Pl 10.78
Basic residues 4
Acidic residues 0
Hydrophobic residues 13
Net charge 4
Boman Index 346
Hydrophobicity 102.5
Aliphatic Index 110
Half Life
Mammalian: 4.4 hour
Yeast: >20 hour
E.coli: >10 hour
Extinction Coefficient cystines 125
Absorbance 280nm 5.43
Polar residues 5
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 33916789
Title Antimicrobial Peptide Brevinin-1RL1 from Frog Skin Secretion Induces Apoptosis and Necrosis of Tumor Cells
Year 2021
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.