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GW-H1
General Information
DRACP ID DRACP01213
Peptide Name GW-H1
Sequence GYNYAKKLANLAKKFANALW
Sequence Length 20
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
Active ACP Induce apoptosis
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| J5 [Human hepatocellular carcinoma] | Hepatocellular carcinoma | Carcinoma | IC50=20.3 μM | MTT assay | 24 h | Patent |
| AGS | Gastric adenocarcinoma | Carcinoma | IC50=17 µM | MTT assay | 24 h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity NIH 3T3: IC50=243 μM
Target Not available
Affinity Not available
Mechanism GW-H1 treatment induced both apoptosis and autophagy of AGS cell line in the early stage, while caspase-dependent apoptosis was further enhanced by inhibition of autophagy at the final stage.
Nature Anticancer; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C109H166N28O26
Absent amino acids CDEHIMPQRSTV
Common amino acids A
Mass 262379
Pl 10.58
Basic residues 4
Acidic residues 0
Hydrophobic residues 10
Net charge 4
Boman Index -1234
Hydrophobicity -34
Aliphatic Index 83.5
Half Life
Mammalian: 30 hour
Yeast: >20 hour
E.coli: >10 hour
Extinction Coefficient cystines 8480
Absorbance 280nm 446.32
Polar residues 6
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 25380626
Title Antimicrobial peptide GW-H1-induced apoptosis of human gastric cancer AGS cell line is enhanced by suppression of autophagy
Year 2015
Patent
Patent ID TWI448297B
Patent Title A method of using novel synthetic peptides for the preparation of drugs with anti-liver cancer activity
Other Iinformation Granted Patent; Family: 2s / 2ex; Family Jurisdictions: TW; Legal Status: Active; Application No: 99121957; Filed: Jul 5, 2010; Published: Aug 11, 2014; Earliest Priority: Jul 5, 2010; Granted: Aug 11, 2014
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.