This work is licensed under a Creative Commons Attribution 4.0 International License.
Apidaecin-1B
General Information
DRACP ID DRACP02050
Peptide Name Apidaecin-1B
Sequence GNNRPVYIPQPRPPHPRL
Sequence Length 18
UniProt ID P35581 Q06601 Q06602 B9UKC1 B9UKC0 B9UKB7 B9UKB5 B9UKB4 Q8WSY8
PubChem CID 102602729
Origin Apis mellifera; Apis cerana cerana
Type Native peptide
Classification
Active ACP Cancer targeted peptides Ribosome inhibitor
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| RAW 264.7 | Mouse macrophage leukemia cell | Leukemia | LC50 =70 µM | XTT assay | 16 h | 1 |
| A431 | Skin squamous cell carcinoma | Carcinoma | 34% Cytotoxicity=10 µM | MTT assay | 24 h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | Carcinoma | Not active up to 10 µM | MTT assay | 24 h | 1 |
Hemolytic Activity Human erythrocytes: 1% Hemolysis=300 µm
Normal (non-cancerous) Cytotoxicity Not available
Target Ribosome
Affinity Not available
Mechanism Proline-rich antimicrobial peptides (PrAMPs) kill bacteria via a non-lytic mechanism in which they permeate through the outer membrane, utilize protein-mediated transport across the inner membrane, and target the ribosome to inhibit protein synthesis.
Nature Anticancer; Antimicrobial
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C95H150N32O23
Absent amino acids ACDEFKMSTW
Common amino acids P
Mass 241182
Pl 12.2
Basic residues 4
Acidic residues 0
Hydrophobic residues 3
Net charge 4
Boman Index -5356
Hydrophobicity -144.44
Aliphatic Index 59.44
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 1490
Absorbance 280nm 87.65
Polar residues 4
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 31209863
Title Identification and elucidation of proline-rich antimicrobial peptides with enhanced potency and delivery
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_1160
This work is licensed under a Creative Commons Attribution 4.0 International License.