This work is licensed under a Creative Commons Attribution 4.0 International License.
Microcin J25
General Information
DRACP ID DRACP02066
Peptide Name Microcin J25
Sequence GGAGHVPEYFVGIGTPISFYG
Sequence Length 21
PubChem CID Not available
Origin Escherichia coli
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| RAW 264.7 | Mouse macrophage leukemia cell | Leukemia | Not active up to 256 µg/ml | MTT assay | 24 h | 1 |
| CaCo-2 | Colon adenocarcinoma | Carcinoma | Not active up to 256 µg/ml | MTT assay | 24 h | 1 |
Hemolytic Activity Mouse erythrocytes: 4.16% Hemolysis=512 µg/ml; Pig erythrocytes: 4.07% Hemolysis=512 µg/ml
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antimicrobial; Antifungal; Anti-Inflammatory
Structure Information
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond LAC: Gly1<--->Glu8
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C101H141N23O28
Absent amino acids CDKLMNQRW
Common amino acids G
Mass 248228
Pl 5.36
Basic residues 1
Acidic residues 1
Hydrophobic residues 7
Net charge 0
Boman Index 1361
Hydrophobicity 40
Aliphatic Index 69.52
Half Life
Mammalian: 100 hour
Yeast: >20 hour
E.coli: >10 hour
Extinction Coefficient cystines 2980
Absorbance 280nm 149
Polar residues 10
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 35250983
Title Biosynthetic Microcin J25 Exerts Strong Antibacterial, Anti-Inflammatory Activities, Low Cytotoxicity Without Increasing Drug-Resistance to Bacteria Target
Year 2022
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_2121
This work is licensed under a Creative Commons Attribution 4.0 International License.