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Piscidin 1
General Information
DRACP ID DRACP02083
Peptide Name Piscidin 1
Sequence FFHHIFRGIVHVGKTIHRLVTG
Sequence Length 22
UniProt ID Q8UUG0
PubChem CID Not available
Origin Morone saxatilis (Striped bass) (Perca saxatilis)
Type Native peptide
Classification
Active ACP
Activity Information
| Cell Line | Disease | Cancer Classified | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|---|
| HT-1080 | Fibrosarcoma | Sarcoma | IC50=5.58 µM | MTT/MTS assay | 24 h, 48 h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | Carcinoma | IC50=5.83 µM | MTT/MTS assay | 24 h, 48 h | 1 |
| A549 | Lung adenocarcinoma | Carcinoma | IC50=4.2 µM | MTT/MTS assay | 24 h, 48 h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Carcinoma | IC50=2.22 µM | MTT/MTS assay | 24 h, 48 h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis=3.12 µg/ml; 100% Hemolysis=25 µg/ml
Normal (non-cancerous) Cytotoxicity HEK293: LC90=50 µg/ml (>90% cells killed)
Target Not available
Affinity Not available
Mechanism Not available
Nature Anticancer; Antimicrobial; Antifungal; Antiviral; Anti-Inflammatory
Structure Information
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C122H187N37O25
Absent amino acids ACDEMNPQSWY
Common amino acids H
Mass 294682
Pl 12.52
Basic residues 7
Acidic residues 0
Hydrophobic residues 10
Net charge 7
Boman Index -1561
Hydrophobicity 45.45
Aliphatic Index 110.45
Half Life
Mammalian: 1.3 hour
Yeast: 3 min
E.coli: 2 min
Extinction Coefficient cystines 0
Absorbance 280nm 0
Polar residues 5
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 34135370
Title Copper-binding anticancer peptides from the piscidin family: an expanded mechanism that encompasses physical and chemical bilayer disruption
Doi 10.1038/s41598-021-91670-w
Year 2021
Literature 2
Pubmed ID 31354312
Title Substitution of lysine for isoleucine at the center of the nonpolar face of the antimicrobial peptide, piscidin-1, leads to an increase in the rapidity of bactericidal activity and a reduction in toxicity
Year 2019
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPR_2370
This work is licensed under a Creative Commons Attribution 4.0 International License.